Proteomics_MS_Insrument	Q Exactive Mass Spectrometer
Proteomics_MS_Instrument_Software_Version	4
Proteomics_Assay_Type	Label Free
Proteomics_MS_Instrument_Sample_Interface	LC
Proteomics_MS_Instrument_Software	Thermo Xcalibur
Proteomics_Assay_Name	DDA
Proteomics_SamplePrep_Protein_Reduction_Agent	DTT
Proteomics_SamplePrep_Digestion_Standard	FALSE
Proteomics_SamplePrep_Peptide_Enrichment	FALSE
Proteomics_SamplePrep_Protein_Fractionation_Mode	N/A
Proteomics_SamplePrep_Lysis_Buffer	"4% SDS, 100 mM Tris-HCl (pH7.6), 1X protease inhibitor"
Proteomics_SamplePrep_Subcellular_Fractionation	FALSE
Proteomics_SamplePrep_Protein_Enrichment_Target	N/A
Proteomics_SamplePrep_Protein_Enrichment	FALSE
Proteomics_SamplePrep_Peptide_Enrichment_Target	N/A
Proteomics_SamplePrep_Digestion_ProteinStandard_AA_Sequence	
Proteomics_SamplePrep_Automation	FALSE
Proteomics_SamplePrep_Subcellular_Fractionation_Target	N/A
Proteomics_SamplePrep_Digestion_Enzyme	Trypsin
Proteomics_SamplePrep_Protein_Fractionation	FALSE
Proteomics_SamplePrep_Peptide_Fractionation_Mode	N/A
Proteomics_SamplePrep_Lysis_Protocol	https://martip03.u.hpc.mssm.edu/pdf/DToxS_SOP_CE-4.0_Drug_Treatment_and_Cell_Lysis.pdf
Proteomics_SamplePrep_Alkylation_Agent	Iodoacetamide
Proteomics_SamplePrep_Digestion_Method	in-gel digestion
Proteomics_SamplePrep_Peptide_Standard_AA_Sequence	
Proteomics_SamplePrep_Sample_Clean_Up	C18 Cartridge
Proteomics_Processing_Analyte_Detection_Mode	Fasta database
Proteomics_Processing_Statistical_Processing_Version	1.6
Proteomics_Processing_Static_Modification_AA	Carbamidomethy C(+57.021454)
Proteomics_Processing_Analyte_Database_Library_File	https://www.uniprot.org/proteomes/UP000005640
Proteomics_Processing_Differential_Modification_AA	oxidation M+15.994915
Proteomics_Processing_Statistical_Quantitation_Processing_Program	https://www.maxquant.org
Proteomics_Processing_Primary_Signal_Processing_Algorithm	Maxquant
Proteomics_Processing_Primary_Signal_Processing_Version	1.6
Proteomics_MS_LC_Instrument	Thermo Scientific UltiMate 3000 RSLC nano LC system
Proteomics_MS_LC_Column	"nano RPLC C18 column (75 um x 50 cm, 2u, 100, Thermo Scientific, P/N 164768)"
Proteomics_LC_Column_Flow_Rate	250
Proteomics_Processing_Statistical_Processing	https://www.maxquant.org
Proteomics_Processing_Database_Search_Engine	Andromeda intergrated into Maxquant
Proteomics_Processing_Database_Search_Engine_Version	1.6
Proteomics_Processing_Data_Filtering_for_Protein	1% FDR
Proteomics_Processing_Data_Filtering_for_Peptide	1% FDR
Proteomics_Fragmentation_Type	HCD
Proteomics_Fragmentation_Gas	N2
Proteomics_Fragmentation_Energy_Spread	Percentage
Proteomics_Mass_Analyzer_MS1	Orbitrap
Proteomics_Mass_Analyzer_MS2	Orbitrap
Proteomics_Mass_Analyzer_Nominal_Resolution_At_MZ400_MS1	140000
Proteomics_Mass_Analyzer_Nominal_Resolution_At_MZ400_MS2	17500
Proteomics_SamplePrep_Peptide_Fractionation	FALSE
