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Dataset Information
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Dataset Name:
Binding affinity of 6 small molecule JAK inhibitors for 5 kinases

Dataset Description:
We tested the binding affinity of 6 small molecule JAK inhibitors (Alvocidib, Nintedanib, Bosutinib, Tofacitinib, Baricitinib, and S-Ruxolitinib)  for JAK and other kinases (JAK1, JAK2, JAK3, TYK2 and DCLK3).

--Data in Package:
20369.csv

--Metadata in Package:
Small_Molecule_Metadata.txt

--Additional Information In Package:
None

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Center-specific Information
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Center-specific Name:
HMS_LINCS

Center-specific Dataset ID:
20369

Center-specific Dataset Link:
http://lincs.hms.harvard.edu/db/datasets/20369/

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Assay Information
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Assay Protocol:
Compounds were sent to Eurofin (DicoverX/Ambit) and binding was tested according to the KdELECT(R) protocol. The KdELECT(R) assay platform is based on a competition binding assay that is run for a compound of interest against a selected panel of  kinases. The assay has three components: a kinase-tagged phage, a test compound, and an immobilized ligand that the compound competes with to displace the kinase. The amount of kinase bound to the immobilized ligand is determined using quantitative PCR of the DNA tag. Results for each kinase are reported as "Kd".

Date Updated:
2019-10-16

Date Retrieved from Center:
11/13/2019

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Metadata Information
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Metadata information regarding the entities used in the experiments is included in the accompanied metadata. A metadata file per entity category is included in the package. For example, the metadata for all the cell lines that were used in the dataset are included in the Cell_Lines_Metadata.txt file.
Descriptions for each metadata field can be found here: http://www.lincsproject.org/data/data-standards/
