################################################################
Dataset Information
################################################################

Dataset Name:
EGF and Insulin combination dose and dynamic response of ERK, AKT, and mTOR signaling and the cell cycle (Cyclin D1) in serum- and growth-factor starved MCF10A cells.

Dataset Description:
MCF10A cells were seeded, allowed to attach overnight in full growth medium, serum-starved for ~24 hours, and then treated with the indicated compound(s) (EGF, Insulin and combinations) for the indicated amount of time (5, 30, 120, 360 min).

--Data Files in Package:
cycD level 1 2018 0626.txt
p4EBP1 level 1 2018 0626.txt
pAkt level 1 2018 0626.txt
pErk level 1 2018 0626.txt

--Metadata Files In Package:
Antibody_Metadata.txt
Cell_Line_Metadata.txt
Map_PCBDataset 2018 0626.txt
Microwestern data template 1.1_PCBDataset 2018 0626.xlsx
Protein_Metadata.txt
Small_Molecule_Metadata.txt

################################################################
Center-specific Information
################################################################

Center-specific Name:
DToxS

Center-specific Dataset ID:
LDS-1523

Center-specific Dataset Link:


################################################################
Assay Information
################################################################

Assay Protocol:
https://martip03.u.hpc.mssm.edu/pdf/DToxS_SOP_A-9.0-Casting_of_Gels_for_Microwestern_Array.pdf; https://martip03.u.hpc.mssm.edu/pdf/DToxS_SOP_A-10.0-Preparation_of_Adherent_Cell_Lysates_for_Microwestern_Array.pdf; https://martip03.u.hpc.mssm.edu/pdf/DToxS_SOP_A-11.0-Printing_of_Samples_for_Microwestern_Array.pdf; https://martip03.u.hpc.mssm.edu/pdf/DToxS_SOP_A-13.0-Gels_Electrophoresis_for_Microwestern_Array.pdf; https://martip03.u.hpc.mssm.edu/pdf/DToxS_SOP_A-14.0-Wet_Transfer_to_Nitrocellulose_for_Microwestern_Array.pdf; https://martip03.u.hpc.mssm.edu/pdf/DToxS_SOP_A-15_0_Antibody%20Incubation_for_Microwestern_Array.pdf; https://martip03.u.hpc.mssm.edu/pdf/DToxS_SOP_A-16_0_Quantifying_Microwestern_Array_Images.pdf

Date Updated:
2018-09-04

Date Retrieved from Center:
8/16/2018

################################################################
Metadata Information
################################################################

Metadata information regarding the entities used in the experiments is included in the accompanied metadata. A metadata file per entity category is included in the package. For example, the metadata for all the cell lines that were used in the dataset are included in the Cell_Lines_Metadata.txt file.
Descriptions for each metadata field can be found here: http://www.lincsproject.org/data/data-standards/
