################################################################
Dataset Information
################################################################

Dataset Name:
cyc D activation dynamics in MCF10A cells treated with the four core compounds.

Dataset Description:
MCF10A cells were seeded, allowed to attach overnight in full growth medium, and then treated with the indicated compound for the indicated amount of time. Cells were lysed, and then assayed for either ERK or Akt activation (phosphorylation). Tubulin signal was used as loading control.

--Data Files in Package:
2017 0307 DrugStudyII data level 3 vers 2018 0405_LINCS.txt

--Metadata Files In Package:
Antibody_Metadata.txt
Cell_Line_Metadata.txt
LDG1401_Experimental_Metadata.xlsx
Small_Molecule_Metadata.txt

################################################################
Center-specific Information
################################################################

Center-specific Name:
DToxS

Center-specific Dataset ID:
LDS-1514

Center-specific Dataset Link:


################################################################
Assay Information
################################################################

Assay Protocol:
https://martip03.u.hpc.mssm.edu/pdf/DToxS_SOP_A-9.0-Casting_of_Gels_for_Microwestern_Array.pdf; https://martip03.u.hpc.mssm.edu/pdf/DToxS_SOP_A-10.0-Preparation_of_Adherent_Cell_Lysates_for_Microwestern_Array.pdf; https://martip03.u.hpc.mssm.edu/pdf/DToxS_SOP_A-11.0-Printing_of_Samples_for_Microwestern_Array.pdf; https://martip03.u.hpc.mssm.edu/pdf/DToxS_SOP_A-13.0-Gels_Electrophoresis_for_Microwestern_Array.pdf; https://martip03.u.hpc.mssm.edu/pdf/DToxS_SOP_A-14.0-Wet_Transfer_to_Nitrocellulose_for_Microwestern_Array.pdf; https://martip03.u.hpc.mssm.edu/pdf/DToxS_SOP_A-15_0_Antibody%20Incubation_for_Microwestern_Array.pdf; https://martip03.u.hpc.mssm.edu/pdf/DToxS_SOP_A-16_0_Quantifying_Microwestern_Array_Images.pdf

Date Updated:

Date Retrieved from Center:
4/13/2018

################################################################
Metadata Information
################################################################

Metadata information regarding the entities used in the experiments is included in the accompanied metadata. A metadata file per entity category is included in the package. For example, the metadata for all the cell lines that were used in the dataset are included in the Cell_Lines_Metadata.txt file.
Descriptions for each metadata field can be found here: http://www.lincsproject.org/data/data-standards/
